(February 18, 2016 at 4:09 pm)FebruaryOfReason Wrote:(February 18, 2016 at 4:02 pm)AAA Wrote: Ok, well then tell me is the formation of a phosphodiester bond a spontaneous reaction? Yup.
https://www.chem.wisc.edu/deptfiles/genc.../dna13.htm
What about the formation of a peptide bond? Standard chemical bond. No god required
Do you know that amino acids have a chiral carbon, meaning they have stereoisomers? Yes I do thanks.
Did you know that only the L form isomer makes proteins? Yes.
What are the odds that a long polypeptide would form with only the L handedness in nature? No odds required. It just does it. Whatever would come out from a D-handed chiral centre is immaterial. We are talking about the product of an L-Chiral center.
Also I am skeptical that you graduated from a University yet you make such an inaccurate statement as saying that DNA can replicate itself. It replicates itself semi-conservatively.
That is just a blatant lie. You need helicases, single stranded binding proteins, DNA polymerases, ligases, topoisomerases, and many more. Also if you formed DNA and proteins in the lab, then congratulations, you should be up for your nobel prize soon, because you are the first one. Except for Meselson and Stahl, ibid. And they basically sat back and watched it happen of it's own accord.
Also if you graduated in chemistry, then you may know about the catalytic efficiency of enzymes. Some of them are literally kinetically perfect, meaning that they catalyze reactions as fast as reactants diffuse and contact them. Have you ever seen a catalyst that reacts that perfectly? Which ones are perfect? Are they really perfect or just very efficient?
No, there is a difference between them forming in nature and being considered spontaneous. Spontaneous have a negative change in free energy, yet peptide bonds do not.
And you are misunderstanding the Meselson and Stahl experiment. It still used the enzymes, they just demonstrated that it was semiconservative rather than conservative, nonconservative, or mixed. Semiconservative just means that each new DNA strand contains one original and one new strand.
And I am holding my biochemistry textbook that says "enzymes that have a kcat/Km ratios at the upper limits have attained kinetic perfection. Their catalytic velocity is restricted only by the rate at which they encounter substrate in the solution." Some that are perfect are acetylcholinesterase, carbonic anydrase, catalase, superoxide dismutase, and there are more listed.
