Evolution in action.

[RoadRunner79]

You might also notice that in addition to the isolated left vs right side, that in each progression, there are multiple lines, that extend on each side, within roughly the same time period.   The more I look at it, the more and more that unguided random mutations, seem unlikely as a cause.

Not sure if we're watching the same video. Yes, there are multiple lines that extend each side, but I don't think you can argue they were "roughly the same time period" when the bacteria on the right side clearly reach each barrier (other than the first) ahead of the left side.

Pause the video at 1:03, 1:15, 1:23, and 1:34 to see what I mean.

Also, I kinda think the initial setup of the experiment with the increasing dosage sizes demonstrates that adaptation was happening. If the original strain of bacteria was already resistant to a 1000x concentration of the antibiotic, why did it keep pausing in growth when it encountered the lower strengths?

I did pause it, in fact; I watched the whole thing paused, using the mouse to scroll through. I think that you are having difficulty distinguishing the tree's from the forest. At best, you have a 1 second pause, in which the previous pane, is becoming more dense. And in watching it this way, the left side actually moves into the 1% solution first, although; it doesn't preform quite as well at times. And in the end, sections of the left are reaching the center at pretty much the same time as the right side. I don't see where you are seeing these huge delays (stops). I'm not sure what you would expect to see, in such a exaggerated selection event. I would expect to see pretty much what was shown. Also, just because they can survive in the antibiotic solution, does not mean that they would prefer to.

You may also note, that this is not the first time, this experiment has been done. The same thing was accomplished by adding antibiotic to a flask in steps by a colleague of Baym, however this makes for a much better video. Also, I'm not saying that this is not evolution. It very well could be change over time, combined with selection. And as I had said, I think from what I had seen in this video, that it would be a fairly repeatable experiment. This would be the evolution that very few would have any contention with.

I think that you are in a pickle Either it is repeatable, and therefore doesn't really demonstrate anything that is a surprise. Or it is not repeatable, in which according to many here, one need not believe or even count as evidence of anything!